Recombinant Biotinylated Chemokine Proteins from ChemoTactics
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Calcium Flux/Mobilization Assay Protocol

Introduction
To test the migration ability of cells in response to the simulation by chemokine. The EC50 of each chemokine will depend on the cell line and receptor.  For example, to test the migration of CXCR4 expressed in Jurkat cells in response to SDF-1a, use 0.003, 0.012, 0.048, 0.019, 0.75. 3.0 nM SDF-1a.

Protocol
  1. Prepare a chemokine concentration gradient in 6 wells of a Transwell plate containing 600ul of growth media.
  2. Add 100ul of a cell suspension at 2.5 million cells/ml to the transwell inserts and place each in the bottom chamber containing the media and chemokine. Incubate for 2 hours at 37C and 5% CO2.
  3. The cells that migrate through the membrane pores to the bottom chamber are counted by flow cytometer.

Questions about this protocol? Contact us and we can help you. 
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Related Wild-Type Chemokine Products:

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  • CCL2 (MCP-1)
  • CCL3 (MIP-1α)
  • CCL4 (MIP-2B)
  • CCL5 (Rantes)











  • CCL7 (MCP-3)
  • CCL14 (HCC-1)
  • CCL19 (MIP-3β)
  • CCL27 (CTACK)
  • CCL28 (MEC)​
  • CXCL8 (IL-8)
  • CXCL10 (IL-10)
  • CXCL12 (SDF-1α)
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​ChemoTactics, Inc
6076 Corte del Cedro Carlsbad,
​CA 92011 USA
Phone: +1-(858)-412-0485
​Email: [email protected]
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  • Chemokine Products
    • Biotinylated Chemokines >
      • Biotinylated CCL2 (MCP-1)
      • Biotinylated CCL3 (MIP-1α)
      • Biotinylated CCL4 (MIP-1β)
      • Biotinylated CCL5 (Rantes)
      • Biotinylated CCL7 (MCP-3)
      • Biotinylated CCL14 (HCC-1)
      • Biotinylated CCL19 (MIP-3β)
      • Biotinylated CCL28 (MEC)
      • Biotinylated CXCL8 (IL-8)
      • Biotinylated CXCL12 (SDF-1α)
    • Unmodified Chemokines >
      • CCL2 (MCP-1)
      • CCL3 (MIP-1α)
      • CCL4 (MIP-1β)
      • CCL5 (RANTES)
      • CCL7 (MCP-3)
      • CCL14 (HCC-1)
      • CCL19 (MIP-3β)
      • CCL27 (CTACK)
      • CCL28 (MEC)
      • CXCL8 (IL-8)
      • CXCL10 (IP-10)
      • CXCL12 (SDF-1α)
  • Streptavidin Conjugates
  • Services
  • Resources
    • Published Applications Biotinylated CXCL12 and CCL5
    • What are Chemokines?
    • Chemokine Protocols >
      • Flow Cytometry with Biotinylated Chemokines
      • ELISA with Biotinylated Chemokines
      • Transwell Migration Assay
    • Blog
  • About
    • Contact