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Chemokines by ChemoTactics

Human Biotin-CCL19 (MIP-3β)
Catalog Number: B-CCL19-2ug, B-CCL19-10ug, B-CCL19-50ug, B-CCL19-100ug  
Lyophilized sample, >97% Purity, EC50 = 15-30nM

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Product Data Sheet (PDF)
Safety Data Sheet (PDF)


​​Standard Products Ship within 1-2 Business Days

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For bulk orders, please email us at info@chemotactics.com for a quote

BACKGROUND
Macrophage inflammatory protein-3-beta (MIP3β) (CCL19), also known as EBI1 ligand chemokine (ELC), directs chemotaxis of dendritic cells, and certain B- and T- lymphocytes, but not monocytes or granulocytes. It is constitutively expressed in thymus and lymph nodes and binds specifically to target cells expressing the receptor CCR7. Being a homeostatic chemokine, its primary physiological role is considered to be in the normal recirculation and homing of lymphocyte. However, MIP-3β could also be proinflammatory, and is implicated in the post-HIV infection responses.


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Also available unlabeled: Wildtype Human CCL19

Biotin-CCL19 (MIP-3β)

  • Size: 1mg
$14,847.00 $11,877.76
Add to Cart

Biotin-CCL19 (MIP-3β)

  • Size: 100ug
$3,270.00 $2,616.00
Add to Cart

Biotin-CCL19 (MIP-3β)

  • Size: 50ug
$1,995.00 $1,564.00
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Biotin-CCL19 (MIP-3β)

  • Size: 10ug
$530.00 $424.00
Add to Cart

Biotin-CCL19 (MIP-3β)

  • Size: 2ug
$130.00 $104.00
Add to Cart

​For bulk orders or custom sizes, please contact us and we can provide this for you.
Custom Sizes
​For bulk orders, please email us at info@chemotactics.com for a quote
SPECIFICATIONS
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Source: E. coli derived Accession # Q99731 (22-98)
Modification: Biotin at C-Terminal​
Formulation: Lyophilized
Carrier Protein: None

Predicted Molecular Mass: 11,218.9807 Da
Extinction Coefficient: 14,180 M-1 cm-1
Actual Molecular Mass: 11,218.9807 Da by ESI Mass Spec

Endotoxin Level:  <0.01 EU per 1μg of the protein by the LAL method
Purity: > 97% by SDS PAGE

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PREPARATION AND STORAGE
Reconstitution: Spin sample prior to reconstitution. Recommended concentration of 100μg/mL in sterile water.
Shipping:  Room Temp

Stability and Storage: Avoid repeated freeze-thaw cycles.
• 12 months from date of receipt, -20 to -70 °C as supplied.
• ChemoTactics suggests using immediately after reconstitution.
• 1 month, -20 to -70 °C under sterile conditions after reconstitution.
Migration Assay: Cells expressing recombinant CCR7 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated MIP-3β. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).
Migration Assay Protocol
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Activity: EC50 = 15-30 nM determined by migration assay with cells expressing recombinant CCR7
REFERENCES
1. “Molecular Cloning of a Novel Human CC Chemokine EBI1-ligand Chemokine That Is a Specific Functional Ligand for EBI1, CCR7”
Yoshida R., Imai T., Hieshima K., Kusuda J., Baba M., Kitaura M., Nishimura M., Kakizaki M., Nomiyama H., Yoshie O.
J Biol Chem 272:13803-13809 (1997)
 
2. "CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells."
Kim C.H., Pelus L.M., White J.R., Applebaum E., Johanson K., Broxmeyer H.E.
J. Immunol. 160:2418-2424(1998)
 
3. “Homeostatic chemokines CCL19 and CCL21 promote inflammation in human immunodeficiency virus-infected patients with ongoing viral replication.”
Damås J.K., Landrø L., Fevang B., Heggelund L., Tjønnfjord G.E., Fløisand Y., Halvorsen B., Frøland S.S., Aukrust P.
Clin Exp Immunol.  157:400-7 (2009)
Other Chemokines
  • ​CCL2 (MCP-1)
  • CCL3 (MIP-1α)
  • CCL4 (MIP-1β)
  • CCL5 (Rantes)
  • CCL7 (MCP-3)
  • CCL14 (HCC-1)
  • CCL19 (MIP-3β)
  • CCL27 (CTACK)
  • CCL28 (MEC)
  • CXCL8 (IL-8)
  • CXCL10 (IP-10)
  • CXCL12 (SDF-1α)
Other Biotinylated Chemokines
  • CCL2 (MCP-1)-Biotin
  • CCL3 (MIP-1α)-Biotin
  • CCL4 (MIP-1B)-Biotin
  • CCL5 (Rantes)-Biotin
  • CCL7 (MCP-3)-Biotin
  • CCL14 (HCC-1)-Biotin
  • CCL19 (MIP-3β)-Biotin
  • CCL28 (MEC)-Biotin
  • CXCL8 (IL-8)-Biotin
  • CXCL12 (SDF-1α)-Biotin
​Support: info@chemotactics.com
For Research use only, Not for use in Humans.
ChemoTactics, Inc. 
6076 Corte Del Cedro
Carlsbad, CA 92011 USA

Phone: (858)-412-0485

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