Human CCL7 (MCP-3)
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BACKGROUND
Monocyte chemotactic protein 3 (MCP-3/CCL7) is secreted by monocytes and certain tumor cell lines. It is a chemoattractant for monocytes and other leukocytes. MCP-3 binds and signals through several chemokine receptors including CCR1, CCR2, CCR3, and appears to be an antagonist for CCR5. Also available biotinylated: Human Biotinylated B-CCL7 |
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SPECIFICATIONS
Source: E. coli derived Accession # P80098 (24-99) Modification: None Formulation: Lyophilized Carrier Protein: None Predicted Molecular Mass: 8.956 kDa Extinction Coefficient: 8,730 M-1 cm-1 Actual Molecular Mass: 8.956 kDa by ESI Mass Spec Protein Sequence: QPVGINTSTTCCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLDKEICADPTQKWV QDFMKHLDKKTQTPKL Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method Purity: > 97% by SDS PAGE |
Migration Assay: Cells expressing recombinant CCR2 were assayed for migration through a transwell filter at various concentrations of MCP-3. Responses are expressed as the % of total input cells.
Migration Assay Protocol 
Activity: EC50 = 0.44 nM determined by migration assay with cells expressing recombinant CCR2
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PREPARATION AND STORAGE
Reconstitution: Spin sample prior to reconstitution. Recommended at 100μg/mL in sterile water
Shipping: Room Temp
Stability and Storage: Spin sample prior to reconstitution. Avoid repeated freeze-thaw cycles
• 12 months from date of receipt, -20 to -70 °C as supplied.
• 1 month, 2 to 8 °C under sterile conditions after reconstitution.
• 3 months, -20 to -70 °C under sterile conditions after reconstitution.
REFERENCES
1. “Monocyte chemotactic protein-3” Menten P., Wuyts A., Van Damme J. Eur. Cytokine Netw. 12:554-560 (2002)
2. "Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines". Opdenakker G., Froyen G., Fiten P., Proost P., Van Damme J. Biochem. Biophys. Res. Commun. 191: 535–42 (1993)
3. "CCR5 binds multiple CC-chemokines: MCP-3 acts as a natural antagonist." Blanpain C., Migeotte I., Lee B., Vakili J., Doranz B.J., Govaerts C., Vassart G., Doms R.W., Parmentier M.Blood 94:1899-1905 (1999)
Reconstitution: Spin sample prior to reconstitution. Recommended at 100μg/mL in sterile water
Shipping: Room Temp
Stability and Storage: Spin sample prior to reconstitution. Avoid repeated freeze-thaw cycles
• 12 months from date of receipt, -20 to -70 °C as supplied.
• 1 month, 2 to 8 °C under sterile conditions after reconstitution.
• 3 months, -20 to -70 °C under sterile conditions after reconstitution.
REFERENCES
1. “Monocyte chemotactic protein-3” Menten P., Wuyts A., Van Damme J. Eur. Cytokine Netw. 12:554-560 (2002)
2. "Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines". Opdenakker G., Froyen G., Fiten P., Proost P., Van Damme J. Biochem. Biophys. Res. Commun. 191: 535–42 (1993)
3. "CCR5 binds multiple CC-chemokines: MCP-3 acts as a natural antagonist." Blanpain C., Migeotte I., Lee B., Vakili J., Doranz B.J., Govaerts C., Vassart G., Doms R.W., Parmentier M.Blood 94:1899-1905 (1999)
