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High-Quality Chemokine Protein Reagents
ChemoTactics produces and validates high-quality recombinant chemokine proteins for research use.
We specialize in producing biotinylated chemokines for various applications and assay design.
We also now provide streptavidin fluorescent conjugates. Contact us for more info.
We also now provide streptavidin fluorescent conjugates. Contact us for more info.
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Validated using strict quality standards
Quality is top priority and separates us from the rest.
We provide fully functional chemokines with the lowest endotoxin levels.
All products are manufactured and tested in US.
We provide fully functional chemokines with the lowest endotoxin levels.
All products are manufactured and tested in US.
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Cells expressing endogenous CXCR4 were stimulated with increasing concentrations of SDF-1α after a 90-minute incubation with calcium dye at 37oC. The maximum response was plotted as a function of the ligand concentration.
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Cells expressing recombinant CCR2 were assayed for migration through a transwell bare filter at various concentrations of MCP-3. The responses are expressed as the % of total input cells.
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Flow Cytometry with Biotinylated Chemokines
A. Uptake of 20nM CXCL12-biotin by U937 cells in the presence (red trace) and absence (cyan) of a CXCR4 inhibitor, AMD 3100. U937 cells are not stained by streptavidin-PE in the absence of CXCL12-biotin (orange).
B. Uptake of 20nM CCL2-biotin by THP1 cells (dark green trace) is abolished by the addition of CCL7 (orange) but not CCL14 (light green), suggesting a CCR2-specific internalization. THP1 cells are not stained in the absence of CCL2-biotin (cyan).
B. Uptake of 20nM CCL2-biotin by THP1 cells (dark green trace) is abolished by the addition of CCL7 (orange) but not CCL14 (light green), suggesting a CCR2-specific internalization. THP1 cells are not stained in the absence of CCL2-biotin (cyan).
Research Testimonials
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Research institutes using our chemokines
CXCR4 X-Ray Crystal Structure PDB: 3ODU
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Cold Spring Harbor Labs, NY, USA
Bristol Myers Squib, USA Caltech, CA USA Istituto Italiano di Tecnologia, Genova, Italy UC San Diego, CA USA Hannover Medical Center, Germany UCSD Moores Cancer Center, CA USA The Scripps Research Institute (TSRI) La Jolla, CA USA University of Piedmont, Italy Regeneron, USA Sanford Consortium for Regenerative Medicine, CA USA Genentech, USA University of Utah, UT USA University of Monash, Australia University of Houston, TX USA UC Berkley, CA USA Tel Aviv University, Israel Pfizer Global R & D, USA University of Georgia, GA USA NIBST, United Kingdom Case Western Reserve University, USA Merck Serono Geneva Research Center, Switzerland Department of Veterans Affairs Medical Center, USA Uppsala University, Sweden Plus Many More |
