|
|
|
ChemoTactics
ChemoTactics produces recombinant chemokine proteins for research use.
We provide affordable prices with flexible sizing available.
We provide affordable prices with flexible sizing available.
What are Chemokines?
|
Chemokines are a class of cytokines responsible for the process of cell movement called chemotaxis. These small secreted signaling proteins act as chemical messengers on G-protein coupled chemokine receptors. In humans, there are over 50 chemokines and 19 chemokine receptors known to date.
As chemokines affect various physiological processes involving cell movement they are vital to our overall health and homeostasis. One important example is maintaining the body's defense mechanisms by guiding immune cells to specific sites in the body where they are needed to combat infections or address tissue damage. |
|
We specialize in producing biotinylated chemokines for various applications and assay design. We also can provide fluorescent chemokines, contact us for more info.
We validate each batch by conducting rigorous testing to ensure quality
|
Cells expressing endogenous CXCR4 were stimulated with increasing concentrations of SDF-1α after a 90-minute incubation with calcium dye at 37oC. The maximum response was plotted as a function of the ligand concentration.
|
Cells expressing recombinant CCR2 were assayed for migration through a transwell bare filter at various concentrations of MCP-3. The responses are expressed as the % of total input cells.
|
Quality is top priority and separates us from the rest.
We provide fully functional chemokines with the lowest endotoxin levels.
All products are manufactured and tested in US.
Flow Cytometry with Biotinylated Chemokines
|
A. Uptake of 20nM CXCL12-biotin by U937 cells in the presence (red trace) and absence (cyan) of a CXCR4 inhibitor, AMD 3100. U937 cells are not stained by streptavidin-PE in the absence of CXCL12-biotin (orange).
|
|
B. Uptake of 20nM CCL2-biotin by THP1 cells (dark green trace) is abolished by the addition of CCL7 (orange) but not CCL14 (light green), suggesting a CCR2-specific internalization. THP1 cells are not stained in the absence of CCL2-biotin (cyan).
|
Create custom assays with biotinylated chemokines
Institutes using our chemokines
CXCR4 X-Ray Crystal Structure
PDB: 3ODU
|
Cold Spring Harbor Labs, NY, USA
Bristol Myers Squib, USA Caltech, CA USA UC San Diego, CA USA Hannover Medical Center, Germany UCSD Moores Cancer Center, CA USA The Scripps Research Institute (TSRI) La Jolla, CA USA University of Piedmont, Italy Regeneron, USA Sanford Consortium for Regenerative Medicine, CA USA Genentech, USA University of Utah, UT USA University of Monash, Australia University of Houston, TX USA UC Berkley, CA USA Tel Aviv University, Israel Pfizer Global R & D, USA University of Georgia, GA USA NIBST, United Kingdom Case Western Reserve University, USA Merck Serono Geneva Research Center, Switzerland Department of Veterans Affairs Medical Center, USA Uppsala University, Sweden Plus Many More |
Fast World-Wide Shipping
|
For in stock items, receive shipments as soon as the next day for US orders or within 2-3 days for international orders. |
|
|
Purchase directly from our product pages or request a quote.
|
ChemoTactics Product Catalog
Product Catalog Contents
Request a catalog by contacting us today
- ChemoTactics company information
- Various types of biotinylated chemokine assays
- Updated product and pricing list
- Detailed product descriptions
- Chemokine assay protocols
- Purchasing conditions and distributor information
Request a catalog by contacting us today
Publications with our Chemokines
- Pisani, A., Donno, R., Valenti, G., Pompa, P. P., Tirelli, N., & Bardi, G. (2022). Chemokine-decorated nanoparticles target specific subpopulations of primary blood mononuclear leukocytes. Nanomaterials, 12(20), 3560. https://doi.org/10.3390/nano12203560
- Pisani, A.; Donno, R.; Gennari, A.; Cibecchini, G.; Catalano, F.; Marotta, R.; Pompa, P.P.; Tirelli, N.; Bardi, G. CXCL12-PLGA/Pluronic Nanoparticle Internalization Abrogates CXCR4-Mediated Cell Migration. Nanomaterials 2020, 10, 2304. https://doi.org/10.3390/nano10112304
- Wang Z, Moresco P, Yan R, Li J, Gao Y, Biasci D, Yao M, Pearson J, Hechtman JF, Janowitz T, Zaidi R, Weiss MJ, Fearon DT. Carcinomas assemble a filamentous CXCL12-keratin-19 coating that suppresses T cell-mediated immune attack. PNAS Jan 2022 Vol. 119 No. 4. https://doi.org/10.1073/pnas.2119463119
- Chatterjee, T., Johnson-Buck, A. Walter, NG. Highly sensitive protein detection by aptamer-based single-molecule kinetic fingerprinting. Biosensors and Bioelectronics. Vol 216 15 Nov 2022. https://doi.org/10.1016/j.bios.2022.114639
- Zhikai Wang, Ran Yan, Jiayun Li, Ya Gao, Philip Moresco, Min Yao, Jaclyn F. Hechtman, Matthew J. Weiss, Tobias Janowitz, Douglas T. Fearon. Pancreatic cancer cells assemble a CXCL12-keratin 19 coating to resist immunotherapy. bioRxiv 776419; doi: https://doi.org/10.1101/776419. Posted: September 4, 2020
- Torretta S., Colombo G., Travelli C, Boumya S., Lim D., Genazzani A., Brolla A. The Cytokine Nicotinamide Phosphoribosyltransferase (eNAMPT; PBEF; Visfatin) Acts as a Natural Antagonist of C-C Chemokine Receptor Type 5 (CCR5). Cells 2020 Feb; 9(2):496. doi 10.3390/cells9020496 PMID: 32098202
- Wu, B., Chien, E.Y., Mol, C.D., Fenalti, G., Liu, W., Katritch, V., Abagyan, R., Brooun, A., Wells, P., Bi, F.C., Hamel, D.J., Kuhn, P., Handel, T.M., Cherezov, V., Stevens, R.C. Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists. Science, 2010. 330: 1066-1071. PMID: 20929726
- Ervin E. Kara, Iain Comerford, Cameron R. Bastow, Kevin A. Fenix, Wendel Litchfield, Tracey M. Handel, and Shaun R. McColl. Distinct chemokine receptor axes regulate T helper 9 cell trafficking to allergic and autoimmune inflammatory sites. J Immunol., 191:1110-1117, 2013. PMID: 23797668
- M. O'Hayre, C.L. Salanga, T.J. Kipps, D. Messmer, P.C. Dorrestein, T.M. Handel (2010) Elucidating the CXCL12/CXCR4 Signaling Network in Chronic Lymphocytic Leukemia through Phosphoproteomic Analysis. PLoS One. 5:e11716. PMID: 20661426
- T.M. Handel, Z. Johnson, M. Mack, R. Cirillo, V. Muzio, M. Teixiera, M. Déruaz, F. Borlat, T.N.C. Wells, and A.E.I. Proudfoot (2008). An Engineered Monomer of CCL2 has Anti-inflammatory Properties Emphasizing the Importance of Oligomerization for Chemokine Activity In Vivo. J Leukocyte Biology. 84(4):1101-8. PMID: 18662971
Contact us at [email protected] or 858-412-0485
for large scale and custom inquiries
Proudly made in the USA
for large scale and custom inquiries
Proudly made in the USA
