Biotinylated Human CCL7 (MCP-3)  
Lyophilized sample, >97% Purity, EC50 = 1.4-2.6nM

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Product Data Sheet (PDF)
Safety Data Sheet (PDF) 


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For bulk orders, please email us at [email protected] for a quote

Also available unlabeled here: Wildtype Human CCL7
BACKGROUND
Monocyte chemotactic protein 3 (MCP-3/CCL7) is secreted by monocytes and certain tumor cell lines. It is a chemoattractant for monocytes and other leukocytes. MCP-3 binds and signals through several chemokine receptors including CCR1, CCR2, CCR3, and appears to be an antagonist for CCR5.

Biotinylated CCL7 is made using the enzymatic method, which has several advantages over chemical biotinylation methods. The attachment of biotin at a specific lysine residue leads to a modified chemokine with functionalities comparable to those of the unmodified CCL7 in migration assay. Combining with avidin analogues conjugated to various fluorescent labels, biotinylated CCL7 is useful in studies on receptor identification, distribution, chemokine binding, and other cellular assays. They serve as great tools in visualization and quantification, and replace the needs for radioactively labeled chemokines.

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Biotin-CCL7 (MCP-3)

$134.00 - $2,862.80
Order Online - Pricing
Catalog Numbers: B-CCL7-2ug, B-CCL7-10ug, B-CCL7-50ug, B-CCL7-100ug​​
Stock Sizes: 2ug, 10ug, 50ug, and 100ug. 
Also available in custom sizes, request a custom quote. 
SPECIFICATIONS

Source: E. coli derived Accession # P80098-2 (24-99)
Modification: Biotin at C-Terminal​
Formulation: Lyophilized
Carrier Protein: None

​Predicted Molecular Mass: 11.374 kDa
Extinction Coefficient: 14,180 M-1 cm-1
Actual Molecular Mass: 11.374 kDa by ESI Mass Spec

Endotoxin Level:  <0.01 EU per 1μg of the protein by the LAL method
Purity: > 97% by SDS PAGE
​

PREPARATION AND STORAGE
Reconstitution: Spin sample prior to resuspension. Recommended at 100μg/mL in sterile water
Shipping: Room Temp

Stability and Storage: Avoid repeated freeze-thaw cycles
• 12 months from date of receipt, -20 to -70 °C as supplied.
• ChemoTactics suggests using immediately after reconstitution.
• 1 month, -20 to -70 °C under sterile conditions after reconstitution.
Migration Assay: Cells expressing recombinant CCR2 were assayed for migration through the Transwell bare filter at various concentrations of MCP-3.  The response is expressed as the % of total input cells (Blue: wild type; Red: biotinylated).
Migration Assay Protocol
​Activity: EC50 = 1.4-2.6nM determined by migration assay with cells expressing recombinant CCR2 cells
​For bulk orders or custom sizes, please contact us and we can provide this for you.
REFERENCES
1. “Monocyte chemotactic protein-3” Menten P., Wuyts A., Van Damme J. Eur. Cytokine Netw. 12:554-560 (2002)

2. "Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines".
Opdenakker G., Froyen G., Fiten P., Proost P., Van Damme J. Biochem. Biophys. Res. Commun. 191: 535–42 (1993)

3. "CCR5 binds multiple CC-chemokines: MCP-3 acts as a natural antagonist." Blanpain C., Migeotte I., Lee B., Vakili J., Doranz B.J., Govaerts C., Vassart G., Doms R.W., Parmentier M.Blood 94:1899-1905 (1999) 

Chemokine Products

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For research use only. Not for use in humans.
Made in the USA