|
Human CCL2 (MCP-1)
Catalog Number: CCL2-5ug, CCL2-20ug, CCL2-50ug, CCL2-100ug, CCL2-1mg Lyophilized sample, >97% Purity, EC50 = 0.03-0.2 nM 
Product Data Sheet (PDF) Safety Data Sheet (PDF) Products ship within 1-2 business days. Also available biotinylated: Human Biotinylated B-CCL2 |
Stock Sizes: 5ug, 20ug, 50ug, 100ug, and 1mg
Also available in custom sizes, email for a custom quote. |
BACKGROUND
Monocyte chemoattractant protein-1 (MCP-1) (CCL2) is produced by many cell types at sites of inflammation. It regulates chemotaxis and transendothielial migration of monocytes, as well as memory T cells and natural killer cells by interacting with their membrane surface receptor CCR2. MCP-1 has also been implicated in a number of disease states, such as rheumatoid arthritis, psoriasis, atherosclerosis, autoimmune diseases, tumor progression, and HIV infection. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It binds to chemokine receptors CCR2 and CCR4.
Monocyte chemoattractant protein-1 (MCP-1) (CCL2) is produced by many cell types at sites of inflammation. It regulates chemotaxis and transendothielial migration of monocytes, as well as memory T cells and natural killer cells by interacting with their membrane surface receptor CCR2. MCP-1 has also been implicated in a number of disease states, such as rheumatoid arthritis, psoriasis, atherosclerosis, autoimmune diseases, tumor progression, and HIV infection. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It binds to chemokine receptors CCR2 and CCR4.
|
SPECIFICATIONS
Source: E. coli derived Accession # P13500 (24-99) Modification: None Formulation: Lyophilized Carrier Protein: None Predicted Molecular Mass: 8.666 kDa Extinction Coefficient: 8,730 M-1 cm-1 Actual Molecular Mass (Mass Spec): 8.666 kDa by ESI Mass Spec Protein Sequence: QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSIDHLDKQTQTPKT Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method Purity: > 97% by SDS PAGE |
Migration Assay: Cells expressing recombinant CCR2 were assayed for migration through a transwell filter at various concentrations of MCP-1. Responses are expressed as the % of total input cells.
Migration Assay Protocol 
Activity: EC50 = 0.066nM determined by Migration Assay with cells expressing recombinant CCR2
|
PREPARATION AND STORAGE
Reconstitution: Spin sample prior to resuspending. Recommended at 100μg/mL in sterile water.
Shipping: Room Temp
Stability and Storage:
Avoid repeated freeze-thaw cycles
• 12 months from date of receipt, -20 to -70 °C as supplied.
• 1 month, 2 to 8 °C under sterile conditions after reconstitution.
• 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution: Spin sample prior to resuspending. Recommended at 100μg/mL in sterile water.
Shipping: Room Temp
Stability and Storage:
Avoid repeated freeze-thaw cycles
• 12 months from date of receipt, -20 to -70 °C as supplied.
• 1 month, 2 to 8 °C under sterile conditions after reconstitution.
• 3 months, -20 to -70 °C under sterile conditions after reconstitution.
REFERENCES
1. “Monocyte Chemoattractant Protein-1 (MCP-1): An Overview” Deshmane S., Kremlev S., Amini S., Sawaya B. J Interferon Cytokine Res. 29: 313–326 (2009)
2. "Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE" Yoshimura T., Yuhki N., Moore S. K., Appella E., Lerman M. I., Leonard E. J. FEBS Letters 244: 487–493 (1989)
3. “Monocyte chemoattractant protein-1: A key mediator in inflammatory processes”. Melgarejo E., Medina M., Sánchez-Jiménez F., Urdiales J. Int J Biochem Cell Biol 41: 998-1001 (2009)
Support: info@chemotactics.com
For Research use only, Not for use in Humans.
