Recombinant Biotinylated Chemokine Proteins from ChemoTactics
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Human CCL2 (MCP-1)
Catalog Number: CCL2-5ug, CCL2-20ug, CCL2-50ug, CCL2-100ug, CCL2-1mg
Lyophilized  sample, >97% Purity, EC50 = 0.03-0.2 nM 
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​Product Data Sheet (PDF)
Safety Data Sheet (PDF)


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Products ship within 1-2 business days. 

​Also available biotinylated: Human Biotinylated B-CCL2

BACKGROUND
Monocyte chemoattractant protein-1 (MCP-1) (CCL2) is produced by many cell types at sites of inflammation. It regulates chemotaxis and transendothielial migration of monocytes, as well as memory T cells and natural killer cells by interacting with their membrane surface receptor CCR2. MCP-1 has also been implicated in a number of disease states, such as rheumatoid arthritis, psoriasis, atherosclerosis, autoimmune diseases, tumor progression, and HIV infection. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It binds to chemokine receptors CCR2 and CCR4.

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CCL2 (MCP-1)

$61.00 - $2,080.00
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​​Stock Sizes: 5ug, 20ug, 50ug, 100ug, and 1mg 
Also available in custom sizes, email for a custom quote. 

SPECIFICATIONS
Source:  E. coli derived Accession # P13500 (24-99)
Modification: None
Formulation: Lyophilized
Carrier Protein: None

Predicted Molecular Mass: 8.666 kDa
Extinction Coefficient: 8,730 M-1 cm-1
Actual Molecular Mass (Mass Spec): 8.666 kDa by ESI Mass Spec

Protein Sequence: QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSIDHLDKQTQTPKT

Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method
Purity: > 97% by SDS PAGE
Migration Assay: Cells expressing recombinant CCR2 were assayed for migration through a transwell filter at various concentrations of MCP-1. Responses are expressed as the % of total input cells.
Migration Assay Protocol
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Activity: EC50 = 0.066nM determined by Migration Assay with cells expressing recombinant CCR2
PREPARATION AND STORAGE
Reconstitution: Spin sample prior to resuspending. Recommended at 100μg/mL in sterile water.
Shipping: Room Temp

Stability and Storage:
Avoid repeated freeze-thaw cycles
             • 12 months from date of receipt, -20 to -70 °C as supplied.
             • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
             • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

REFERENCES
1. “Monocyte Chemoattractant Protein-1 (MCP-1): An Overview” Deshmane S., Kremlev S., Amini S., Sawaya B. J Interferon Cytokine Res. 29: 313–326 (2009)

2. "Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE" Yoshimura T., Yuhki N., Moore S. K., Appella E., Lerman M. I., Leonard E. J. FEBS Letters 244: 487–493 (1989)

3. “Monocyte chemoattractant protein-1: A key mediator in inflammatory processes”. Melgarejo E., Medina M., Sánchez-Jiménez F., Urdiales J. Int J Biochem Cell Biol 41: 998-1001 (2009)

​For bulk orders or custom sizes, please contact us and we can provide this for you.
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Chemokine Products

Unlabeled Chemokines
  • ​CCL2 (MCP-1)
  • CCL3 (MIP-1a)
  • CCL4 (MIP-1B)
  • CCL5 (Rantes)
  • CCL7 (MCP-3)
  • CCL14 (HCC-1)
  • CCL19 (MIP-3B)
  • CCL27 (CTACK)
  • CCL28 (MEC)
  • CXCL8 (IL-8)
  • CXCL10 (IP-10)
  • CXCL12 (SDF-1a)
Biotinylated Chemokines
  • CCL2 (MCP-1)-Biotin
  • CCL3 (MIP-1a)-Biotin
  • CCL4 (MIP-1B)-Biotin
  • CCL5 (Rantes)-Biotin
  • CCL7 (MCP-3)-Biotin
  • CCL14 (HCC-1)-Biotin
  • CCL19 (MIP-3B)-Biotin
  • CCL28 (MEC)-Biotin
  • CXCL8 (IL-8)-Biotin
  • CXCL12 (SDF-1a)-Biotin

Support: [email protected]
For Research use only, Not for use in Humans.

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​ChemoTactics, Inc
6076 Corte del Cedro Carlsbad,
​CA 92011 USA
Phone: +1-(858)-412-0485
​Email: [email protected]
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  • Chemokine Products
    • Biotinylated Chemokines >
      • Biotinylated CCL2 (MCP-1)
      • Biotinylated CCL3 (MIP-1α)
      • Biotinylated CCL4 (MIP-1β)
      • Biotinylated CCL5 (Rantes)
      • Biotinylated CCL7 (MCP-3)
      • Biotinylated CCL14 (HCC-1)
      • Biotinylated CCL19 (MIP-3β)
      • Biotinylated CCL28 (MEC)
      • Biotinylated CXCL8 (IL-8)
      • Biotinylated CXCL12 (SDF-1α)
    • Unmodified Chemokines >
      • CCL2 (MCP-1)
      • CCL3 (MIP-1α)
      • CCL4 (MIP-1β)
      • CCL5 (RANTES)
      • CCL7 (MCP-3)
      • CCL14 (HCC-1)
      • CCL19 (MIP-3β)
      • CCL27 (CTACK)
      • CCL28 (MEC)
      • CXCL8 (IL-8)
      • CXCL10 (IP-10)
      • CXCL12 (SDF-1α)
  • Streptavidin Conjugates
  • Services
  • Resources
    • Published Applications Biotinylated CXCL12 and CCL5
    • What are Chemokines?
    • Chemokine Protocols >
      • Flow Cytometry with Biotinylated Chemokines
      • ELISA with Biotinylated Chemokines
      • Transwell Migration Assay
    • Blog
  • About
    • Contact