Human Biotin-CCL14 (HCC-1)
Lyophilized sample, >97% Purity, EC50 = 0.2-0.4nM

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Product Data Sheet (PDF)
Safety Data Sheet (PDF)


Standard Products Ship within 1-2 Business Days
For bulk orders, please email us at [email protected] for a quote​

​Also available unlabeled here: Wildtype Human CCL14
BACKGROUND
Hemofiltrate CC chemokine-1(HCC-1)(CCL14) is constitutively expressed by numerous tissues. Upon processing of the N terminal residues of the full length HCC-1 by the uPA-plasmin system, the active form of HCC-1 (made by ChemoTactics) is a strong agonist for CCR1, CCR5 and to a lesser extent CCR3, and causes chemotaxis of different types of leukocytes. The active form of HCC-1 is also shown as a potent inhibitor of HIV entry.
Catalog Numbers: B-CCL14-2ug, B-CCL14-10ug, B-CCL14-50ug, B-CCL14-100ug  ​​
Stock Sizes: 2ug, 10ug, 50ug, and 100ug.
Also available in custom sizes, request a custom quote. 

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Biotin-CCL14 (HCC-1)

$134.00 - $2,862.80
Order Online - Pricing
SPECIFICATIONS

Source: E. coli derived Accession # Q16627 (28-93)
Modification: Biotin at C-Terminal​
Formulation: Lyophilized
Carrier Protein: None


Predicted Molecular Mass: 10,219.5471 Da
Extinction Coefficient: 19,300 M-1 cm-1
Actual Molecular Mass: 10,219.5471 Da by ESI Mass Spec

Endotoxin Level:  <0.01 EU per 1μg of the protein by the LAL method
Purity: > 97% by SDS PAGE

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PREPARATION AND STORAGE
Reconstitution: Spin sample prior to reconstitution. Recommended concentration of 100μg/mL in sterile water.
Shipping:  Room Temp

Stability and Storage: Avoid repeated freeze-thaw cycles.
• 12 months from date of receipt, -20 to -70 °C as supplied.
• ChemoTactics suggests using immediately after reconstitution.
• 1 month, -20 to -70 °C under sterile conditions after reconstitution.
​Migration Assay: Cells expressing recombinant CCR1 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated HCC-1. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).
Migration Assay protocol
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​Activity: EC50 = 0.2-0.4 nM determined by migration assay with cells expressing recombinant CCR1
​For bulk orders or custom sizes, please contact us and we can provide this for you.
REFERENCES
​1. “HCC-1, a novel chemokine from human plasma” Schulz-Knappe P.,  Mägert H., Dewald  B., J Exp Med 183:295-299 (1996)

2. “Urokinase Plasminogen Activator and Plasmin Efficiently Convert Hemofiltrate CC Chemokine 1 into Its Active [9–74] Processed Variant” Vakili J., Standker L., Detheux M., Vassart, G., Forssmann W., Parmentier M. J Immunol 167:3406-3413 (2001)
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Chemokine Products

​Support: [email protected]
For Research use only, Not for use in Humans.
Made in the USA