Human Biotin-CCL14 (HCC-1)
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Stock Sizes: 2ug, 10ug, 50ug, 100ug, and 1mg
Also available in custom sizes, email for a custom quote. |
SPECIFICATIONS
Source: E. coli derived Accession # Q16627 (28-93) Modification: Biotin at C-Terminal Formulation: Lyophilized Carrier Protein: None Predicted Molecular Mass: 10,219.5471 Da Extinction Coefficient: 19,300 M-1 cm-1 Actual Molecular Mass: 10,219.5471 Da by ESI Mass Spec Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method Purity: > 97% by SDS PAGE PREPARATION AND STORAGE Reconstitution: Spin sample prior to reconstitution. Recommended concentration of 100μg/mL in sterile water. Shipping: Room Temp Stability and Storage: Avoid repeated freeze-thaw cycles. • 12 months from date of receipt, -20 to -70 °C as supplied. • ChemoTactics suggests using immediately after reconstitution. • 1 month, -20 to -70 °C under sterile conditions after reconstitution. |
Migration Assay: Cells expressing recombinant CCR1 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated HCC-1. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).
Migration Assay protocol Activity: EC50 = 0.2-0.4 nM determined by migration assay with cells expressing recombinant CCR1
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For bulk orders or custom sizes, please contact us and we can provide this for you.
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REFERENCES
1. “HCC-1, a novel chemokine from human plasma” Schulz-Knappe P., Mägert H., Dewald B., J Exp Med 183:295-299 (1996)
2. “Urokinase Plasminogen Activator and Plasmin Efficiently Convert Hemofiltrate CC Chemokine 1 into Its Active [9–74] Processed Variant” Vakili J., Standker L., Detheux M., Vassart, G., Forssmann W., Parmentier M. J Immunol 167:3406-3413 (2001)
1. “HCC-1, a novel chemokine from human plasma” Schulz-Knappe P., Mägert H., Dewald B., J Exp Med 183:295-299 (1996)
2. “Urokinase Plasminogen Activator and Plasmin Efficiently Convert Hemofiltrate CC Chemokine 1 into Its Active [9–74] Processed Variant” Vakili J., Standker L., Detheux M., Vassart, G., Forssmann W., Parmentier M. J Immunol 167:3406-3413 (2001)
Support: [email protected]
For Research use only, Not for use in Humans.
For Research use only, Not for use in Humans.