Human CXCL8 (IL-8)
Cat Number: CXCL8-5ug, CXCL8-20ug, CXCL8-50ug, CXCL8-100ug, CXCL8-1mg
Lyophilized sample, >97% Purity, EC50 = 0.083nM

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​Also available biotinylated: Human Biotinylated B-CXCL8
BACKGROUND
Interleukin 8 (IL-8 /CXCL8) is secreted primarily by macrophages and monocytes.  It is one of the key mediators for inflammatory responses. IL-8 is a strong chemotractant for neutrophils and monocytes promoting activation of these cells by binding to two cell surface receptors, CXCR1 and CXCR2. It is also a strong angiogenic agent and is considered to play a role in the pathogenesis of bronchiolitis.

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CXCL8 (IL-8)

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​​Stock Sizes: 5ug, 20ug, 50ug, 100ug, and 1mg 
Also available in custom sizes, email for a custom quote. 
SPECIFICATIONS
Source: E. coli derived Accession # P10145 (28-99)
Modification: None
Formulation: Lyophilized
Carrier Protein: None
Predicted Molecular Mass: 8.386 kDa
Extinction Coefficient: 7,450 M-1 cm-1
Actual Molecular Mass: 8.386 kDa by ESI Mass Spec
Protein Sequence: SAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKFLKRAEN
Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method
Purity: > 97% by SDS PAGE
PREPARATION AND STORAGE
Reconstitution: Spin tube prior to resuspension. Recommended at 100μg/mL in sterile water
Shipping: Room Temp

Stability and Storage:  Avoid repeated freeze-thaw cycles
             • 12 months from date of receipt, -20 to -70 °C as supplied.
             • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
             • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Migration Assay: Cells expressing recombinant CXCR1 were assayed for migration through a transwell filter at various concentrations of WT IL-8. Responses are expressed as the % of total input cells.
Migration Assay Protocol
Activity: EC50 = 0.083 nM determined by migration assay with cells expressing recombinant CXCR1 ​
REFERENCES
1. “Interleukin-8, a chemotactic and inflammatory cytokine” Baggiolini M., Clark-Lewis I. FEBS Lett. 307:97-101(1992)

2.  “Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction MDNCF mRNA by interleukin 1 and tumor necrosis factor” Matsushima K., Morishita K., Yoshimura T., Lavu S., Kobayashi Y., Lew W., Appella E., Kung H., Leonard E.J., Oppenheim J.J. J. Exp. Med. 167:1883-1893(1988)

3. “Chemokines, CXC|IL-8” Strieter R.M., Keane M.P., Belperio J. A. Encyclopedia of respiratory medicine, Academic Press, Oxford, P395-398(2006)
For bulk orders or custom sizes, please contact us and we can provide this for you.

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For Research use only, Not for use in Humans.

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