Biotinylated Chemokines
Flexible assay development for many applications; including flow cytometry, binding assays, and various fluorescent detection.
Our recombinant system has been optimized to ensure endogeneous functional activity. We diligently test our products to ensure their activity and report their specifications.
Activity Assays - Calcium Flux and Cell Migration
U937 cells expressing endogenous CXCR4 were stimulated with increasing concentrations of SDF-1α after a 90-minute incubation with calcium dye at 37oC. The maximum response was plotted as a function of the ligand concentration (Red: wild type; Blue: biotinylated).
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Cells expressing recombinant CCR2 were assayed for migration through a transwell bare filter at various concentrations of MCP-3. The responses are expressed as the % of total input cells (Red: wild type; Blue: biotinylated).
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Flow Cytometry Assays
A. Uptake of CXCL12-biotin by U937 cells in the presence (red trace) and absence (cyan) of a CXCR4 inhibitor, AMD 3100. U937 cells are not stained by streptavidin-PE in the absence of CXCL12-biotin (orange).
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B. Uptake of CCL2-biotin by THP1 cells (dark green trace) is abolished by the addition of CCL7 (orange) but not CCL14 (light green), suggesting a CCR2-specific internalization. THP1 cells are not stained in the absence of CCL2-biotin (cyan).
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Imaging Applications - Fluorescent Cell Surface Detection
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Visualization of GAG Binding
Detection of chemokine GAG binding on the cell surface using increasing amounts of biotinylated CCL5. |
Western Detection of IL-8
Detection of various amounts of biotinylated CXCL8 (IL-8) using streptavidin conjugated to 800W dye.
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Temperature Dependent CXCR4 Receptor Internalization
Distribution of the endogenous CXCR4 in Jurkat cells at 4°C (left image) and their internalization upon stimulation at 37°C (right image) were revealed by the biotinylated SDF-1α bound by streptavidin-Cy3 (red).
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4°C
In presence of biotinylated SDF-1α bound by streptavidin-Cy3 (red), the majority of the CXCR4 population is located at the plasma membrane at 4 C.
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37°C
The majority of the population of CXCR4 at 37 C in the presence of biotinylated SDF-1α bound by streptavidin-Cy3 (red) has been internalized.
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Biotinylated Chemokine Products
Human CCL2 (MCP-1) Biotinylated
Lyophilized sample, >97% Purity, EC50 = 0.25-1.2nM 2 µg - $130 10 µg - $530 50 µg - $1,955 100 µg - $3,270 |
Human CCL3 (MIP-1α) Biotinylated
Lyophilized sample, >97% Purity, EC50 = 1.1-2.6nM 2 µg - $130 10 µg - $530 50 µg - $1,955 100 µg - $3,270 |
Human CCL4 (MIP-1β) Biotinylated
Lyophilized sample, >97% Purity, EC50 = 0.3-1.2nM 2 µg - $130 10 µg - $530 50 µg - $1,955 100 µg - $3,270 |
Human CCL5 (Rantes) Biotinylated
Lyophilized sample, >97% Purity, EC50 = 0.25-0.50nM 2 µg - $130 10 µg - $530 50 µg - $1,955 100 µg - $3,270 |
Human CCL7 (MCP-3) Biotinylated
Lyophilized sample, >97% Purity, EC50 = 1.4-2.6nM 2 µg - $130 10 µg - $530 50 µg - $1,955 100 µg - $3,270 |
Human CCL14 (HCC-1) Biotinylated
Lyophilized sample, >97% Purity, EC50 = 0.2-0.4nM 2 µg - $130 10 µg - $530 50 µg - $1,955 100 µg - $3,270 |
Human CCL19 (MIP-3β) Biotinylated
Lyophilized sample, >97% Purity, EC50 = 15-30nM 2 µg - $130 10 µg - $530 50 µg - $1,955 100 µg - $3,270 |
Human CCL28 (MEC) Biotinylated
Lyophilized sample, >97% Purity, EC50 = 40-60nM 2 µg - $130 10 µg - $530 50 µg - $1,955 100 µg - $3,270 |
Human CXCL8 (IL-8) Biotinylated
Lyophilized sample, >97% Purity, EC50 = 2.5nM 2 µg - $130 10 µg - $530 50 µg - $1,955 100 µg - $3,270 |
Human CXCL12 (SDF-1α) Biotinylated
Lyophilized sample, >97% Purity, EC50 = 2.5nM 2 µg - $130 10 µg - $530 50 µg - $1,955 100 µg - $3,270 |
