Recombinant Biotinylated Chemokine Proteins from ChemoTactics
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Biotinylated Human CCL2 (MCP-1)

Catalog Numbers: B-CCL2-2ug, B-CCL2-10ug, B-CCL2-50ug, B-CCL2-100ug 
B-CCL2
Stock Sizes: 2ug, 10ug, 50ug, 100ug, and 1mg 
Lyophilized sample, >97% Purity, EC50 = 1.26nM ​
Standard sizes ship within 1-2 business days
Email [email protected] for a custom size quote

BACKGROUND

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is produced by many cell types at sites of inflammation. It regulates chemotaxis and transendothelial migration of monocytes, as well as memory T cells and natural killer cells by interacting with their membrane surface receptor CCR2. MCP-1 has also been implicated in a number of disease states, such as rheumatoid arthritis, atherosclerosis, autoimmune diseases, tumor progression, and HIV infection. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It binds to chemokine receptors CCR2 and CCR4.

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Biotin-CCL2 (MCP-1)

$134.00 - $2,862.80
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Stock Sizes:
 2ug, 10ug, 50ug, 100ug, and 1mg 
​Catalog Numbers: B-CCL2-2ug, B-CCL2-10ug, B-CCL2-50ug, B-CCL2-100ug ​
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SPECIFICATIONS

Source: E. coli derived Accession # P13500-2 (24-99)
Modification: Biotin at C-Terminal
Formulation: Lyophilized
Carrier Protein: None
Predicted Molecular Mass: 11.085 kDa
Extinction Coefficient: 14,180 M-1 cm-1
Actual Molecular Mass: 11.085 kDa by ESI MS
Endotoxin Level:  <0.01 EU per 1μg of the protein by the LAL method
Purity: > 97% by SDS PAGE
PREPARATION AND STORAGE

​Reconstitution: Spin sample prior to reconstitution. Recommended concentration of 100μg/mL in sterile water.
Shipping:  Room Temp

​Stability and Storage
: Avoid repeated freeze-thaw cycles. 
• 12 months from date of receipt, -20 to -70 °C as supplied.
• ChemoTactics suggests using immediately after reconstitution.
• 1 month, -20 to -70 °C under sterile conditions after reconstitution.

Flow Cytometry:  Uptake of biotin-CCL2 by THP1 cells (dark green) is abolished by the addition of  CCL7 (orange) but not CCL14 (light green), suggesting a CCR2-specific internalization.  THP1 cells are not stained in the absence of biotin-CCL2-biotin (cyan).
Flow cytometry protocol
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20nM Biotinylated CCL2 with Streptavidin PE on THP-1 Cells 
Migration Assay: Cells expressing recombinant CCR2 were assayed for migration through a transwell filter at various concentrations of MCP-1. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).
Migration Assay protocol
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Activity: EC50 = 1.26nM determined by migration assay of THP-1 Cells

Documentation

  • Product Data Sheet (PDF)
  • Safety Data Sheet (PDF)

​Also available unlabeled:
 Wildtype Human CCL2

REFERENCES
1. “Monocyte Chemoattractant Protein-1 (MCP-1): An Overview” Deshmane S.,  Kremlev S.,  Amini S., Sawaya B. J Interferon Cytokine Res. 29: 313–326 (2009)
 
2. "Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE" Yoshimura T., Yuhki N., Moore S. K., Appella E., Lerman M. I., Leonard E. J.  FEBS Letters 244: 487–493 (1989)
 
3. “Monocyte chemoattractant protein-1: A key mediator in inflammatory processes” Melgarejo E., Medina M., Sánchez-Jiménez F., Urdiales J. Int J Biochem Cell Biol 41: 998-1001 (2009)

Chemokine Products

Unlabeled Chemokines
  • ​CCL2 (MCP-1)
  • CCL3 (MIP-1a)
  • CCL4 (MIP-1B)
  • CCL5 (Rantes)
  • CCL7 (MCP-3)
  • CCL14 (HCC-1)
  • CCL19 (MIP-3B)
  • CCL27 (CTACK)
  • CCL28 (MEC)
  • CXCL8 (IL-8)
  • CXCL10 (IP-10)
  • CXCL12 (SDF-1a)​
Biotinylated Chemokines
  • CCL2 (MCP-1)-Biotin
  • CCL3 (MIP-1a)-Biotin
  • CCL4 (MIP-1B)-Biotin
  • CCL5 (Rantes)-Biotin
  • CCL7 (MCP-3)-Biotin
  • CCL14 (HCC-1)-Biotin
  • CCL19 (MIP-3B)-Biotin
  • CCL28 (MEC)-Biotin
  • CXCL8 (IL-8)-Biotin
  • CXCL12 (SDF-1a)-Biotin​
​Support: [email protected]
For Research use only, Not for use in Humans.
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​ChemoTactics, Inc
6076 Corte del Cedro Carlsbad,
​CA 92011 USA
Phone: +1-(858)-412-0485
​Email: [email protected]
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Made in the USA
  • Chemokine Products
    • Biotinylated Chemokines >
      • Biotinylated CCL2 (MCP-1)
      • Biotinylated CCL3 (MIP-1α)
      • Biotinylated CCL4 (MIP-1β)
      • Biotinylated CCL5 (Rantes)
      • Biotinylated CCL7 (MCP-3)
      • Biotinylated CCL14 (HCC-1)
      • Biotinylated CCL19 (MIP-3β)
      • Biotinylated CCL28 (MEC)
      • Biotinylated CXCL8 (IL-8)
      • Biotinylated CXCL12 (SDF-1α)
    • Unmodified Chemokines >
      • CCL2 (MCP-1)
      • CCL3 (MIP-1α)
      • CCL4 (MIP-1β)
      • CCL5 (RANTES)
      • CCL7 (MCP-3)
      • CCL14 (HCC-1)
      • CCL19 (MIP-3β)
      • CCL27 (CTACK)
      • CCL28 (MEC)
      • CXCL8 (IL-8)
      • CXCL10 (IP-10)
      • CXCL12 (SDF-1α)
  • Streptavidin Conjugates
  • Services
  • Resources
    • Published Applications Biotinylated CXCL12 and CCL5
    • What are Chemokines?
    • Chemokine Protocols >
      • Flow Cytometry with Biotinylated Chemokines
      • ELISA with Biotinylated Chemokines
      • Transwell Migration Assay
    • Blog
  • About
    • Contact