Biotinylated Human CCL19 (MIP-3β)
Lyophilized sample, >97% Purity, EC50 = 15-30nM

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Product Data Sheet (PDF)
Safety Data Sheet (PDF)


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For bulk orders, please email us at [email protected] for a quote

​Also available unlabeled: Wildtype Human CCL19
BACKGROUND
Macrophage inflammatory protein-3-beta (MIP3β) (CCL19), also known as EBI1 ligand chemokine (ELC), directs chemotaxis of dendritic cells, and certain B- and T- lymphocytes, but not monocytes or granulocytes. It is constitutively expressed in thymus and lymph nodes and binds specifically to target cells expressing the receptor CCR7. Being a homeostatic chemokine, its primary physiological role is considered to be in the normal recirculation and homing of lymphocyte. However, MIP-3β could also be proinflammatory, and is implicated in the post-HIV infection responses.

On Sale

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Biotin-CCL19 (MIP-3β)

$134.00 - $2,862.80
Order Online - Pricing
Catalog Numbers: B-CCL19-2ug, B-CCL19-10ug, B-CCL19-50ug, B-CCL19-100ug  ​​
Stock Sizes: 2ug, 10ug, 50ug, and 100ug. 
Also available in custom sizes, request a custom quote. 
SPECIFICATIONS
​
Source: E. coli derived Accession # Q99731 (22-98)
Modification: Biotin at C-Terminal​
Formulation: Lyophilized
Carrier Protein: None

Predicted Molecular Mass: 11,218.9807 Da
Extinction Coefficient: 14,180 M-1 cm-1
Actual Molecular Mass: 11,218.9807 Da by ESI Mass Spec

Endotoxin Level:  <0.01 EU per 1μg of the protein by the LAL method
Purity: > 97% by SDS PAGE

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PREPARATION AND STORAGE
Reconstitution: Spin sample prior to reconstitution. Recommended concentration of 100μg/mL in sterile water.
Shipping:  Room Temp

Stability and Storage: Avoid repeated freeze-thaw cycles.
• 12 months from date of receipt, -20 to -70 °C as supplied.
• ChemoTactics suggests using immediately after reconstitution.
• 1 month, -20 to -70 °C under sterile conditions after reconstitution.
Migration Assay: Cells expressing recombinant CCR7 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated MIP-3β. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).
Migration Assay Protocol
Activity: EC50 = 15-30 nM determined by migration assay with cells expressing recombinant CCR7
​For bulk orders or custom sizes, please contact us and we can provide this for you.
REFERENCES
1. “Molecular Cloning of a Novel Human CC Chemokine EBI1-ligand Chemokine That Is a Specific Functional Ligand for EBI1, CCR7”
Yoshida R., Imai T., Hieshima K., Kusuda J., Baba M., Kitaura M., Nishimura M., Kakizaki M., Nomiyama H., Yoshie O.
J Biol Chem 272:13803-13809 (1997)
 
2. "CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells."
Kim C.H., Pelus L.M., White J.R., Applebaum E., Johanson K., Broxmeyer H.E.
J. Immunol. 160:2418-2424(1998)
 
3. “Homeostatic chemokines CCL19 and CCL21 promote inflammation in human immunodeficiency virus-infected patients with ongoing viral replication.”
Damås J.K., Landrø L., Fevang B., Heggelund L., Tjønnfjord G.E., Fløisand Y., Halvorsen B., Frøland S.S., Aukrust P.
Clin Exp Immunol.  157:400-7 (2009)

Chemokine Products

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For Research use only, Not for use in Humans.
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