Recombinant Biotinylated Chemokine Proteins from ChemoTactics
  • Chemokine Products
    • Biotinylated Chemokines >
      • Biotinylated CCL2 (MCP-1)
      • Biotinylated CCL3 (MIP-1α)
      • Biotinylated CCL4 (MIP-1β)
      • Biotinylated CCL5 (Rantes)
      • Biotinylated CCL7 (MCP-3)
      • Biotinylated CCL14 (HCC-1)
      • Biotinylated CCL19 (MIP-3β)
      • Biotinylated CCL28 (MEC)
      • Biotinylated CXCL8 (IL-8)
      • Biotinylated CXCL12 (SDF-1α)
    • Unmodified Chemokines >
      • CCL2 (MCP-1)
      • CCL3 (MIP-1α)
      • CCL4 (MIP-1β)
      • CCL5 (RANTES)
      • CCL7 (MCP-3)
      • CCL14 (HCC-1)
      • CCL19 (MIP-3β)
      • CCL27 (CTACK)
      • CCL28 (MEC)
      • CXCL8 (IL-8)
      • CXCL10 (IP-10)
      • CXCL12 (SDF-1α)
  • Streptavidin Conjugates
  • Services
  • Resources
    • Published Applications Biotinylated CXCL12 and CCL5
    • What are Chemokines?
    • Chemokine Protocols >
      • Flow Cytometry with Biotinylated Chemokines
      • ELISA with Biotinylated Chemokines
      • Transwell Migration Assay
    • Blog
  • About
    • Contact

Biotinylated Human CCL19 (MIP-3β)
Lyophilized sample, >97% Purity, EC50 = 15-30nM

Picture
Product Data Sheet (PDF)
Safety Data Sheet (PDF)


​​Standard Products Ship within 1-2 Business Days
For bulk orders, please email us at [email protected] for a quote

​Also available unlabeled: Wildtype Human CCL19

BACKGROUND
Macrophage inflammatory protein-3-beta (MIP3β) (CCL19), also known as EBI1 ligand chemokine (ELC), directs chemotaxis of dendritic cells, and certain B- and T- lymphocytes, but not monocytes or granulocytes. It is constitutively expressed in thymus and lymph nodes and binds specifically to target cells expressing the receptor CCR7. Being a homeostatic chemokine, its primary physiological role is considered to be in the normal recirculation and homing of lymphocyte. However, MIP-3β could also be proinflammatory, and is implicated in the post-HIV infection responses.

On Sale

On Sale

Biotin-CCL19 (MIP-3β)

$134.00 - $2,862.80
Order Online - Pricing
Catalog Numbers: B-CCL19-2ug, B-CCL19-10ug, B-CCL19-50ug, B-CCL19-100ug  ​​
Stock Sizes: 2ug, 10ug, 50ug, and 100ug. 
Also available in custom sizes, request a custom quote. 
Request a Quote

SPECIFICATIONS
​
Source: E. coli derived Accession # Q99731 (22-98)
Modification: Biotin at C-Terminal​
Formulation: Lyophilized
Carrier Protein: None

Predicted Molecular Mass: 11,218.9807 Da
Extinction Coefficient: 14,180 M-1 cm-1
Actual Molecular Mass: 11,218.9807 Da by ESI Mass Spec

Endotoxin Level:  <0.01 EU per 1μg of the protein by the LAL method
Purity: > 97% by SDS PAGE

​
PREPARATION AND STORAGE
Reconstitution: Spin sample prior to reconstitution. Recommended concentration of 100μg/mL in sterile water.
Shipping:  Room Temp

Stability and Storage: Avoid repeated freeze-thaw cycles.
• 12 months from date of receipt, -20 to -70 °C as supplied.
• ChemoTactics suggests using immediately after reconstitution.
• 1 month, -20 to -70 °C under sterile conditions after reconstitution.
Migration Assay: Cells expressing recombinant CCR7 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated MIP-3β. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).
Migration Assay Protocol
Picture
Activity: EC50 = 15-30 nM determined by migration assay with cells expressing recombinant CCR7
​For bulk orders or custom sizes, please contact us and we can provide this for you.
Custom Sizes
REFERENCES
1. “Molecular Cloning of a Novel Human CC Chemokine EBI1-ligand Chemokine That Is a Specific Functional Ligand for EBI1, CCR7”
Yoshida R., Imai T., Hieshima K., Kusuda J., Baba M., Kitaura M., Nishimura M., Kakizaki M., Nomiyama H., Yoshie O.
J Biol Chem 272:13803-13809 (1997)
 
2. "CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells."
Kim C.H., Pelus L.M., White J.R., Applebaum E., Johanson K., Broxmeyer H.E.
J. Immunol. 160:2418-2424(1998)
 
3. “Homeostatic chemokines CCL19 and CCL21 promote inflammation in human immunodeficiency virus-infected patients with ongoing viral replication.”
Damås J.K., Landrø L., Fevang B., Heggelund L., Tjønnfjord G.E., Fløisand Y., Halvorsen B., Frøland S.S., Aukrust P.
Clin Exp Immunol.  157:400-7 (2009)

Chemokine Products

Unlabeled Chemokines
  • ​CCL2 (MCP-1)
  • CCL3 (MIP-1a)
  • CCL4 (MIP-1B)
  • CCL5 (Rantes)
  • CCL7 (MCP-3)
  • CCL14 (HCC-1)
  • CCL19 (MIP-3B)
  • CCL27 (CTACK)
  • CCL28 (MEC)
  • CXCL8 (IL-8)
  • CXCL10 (IP-10)
  • CXCL12 (SDF-1a)​
Biotinylated Chemokines
  • CCL2 (MCP-1)-Biotin
  • CCL3 (MIP-1a)-Biotin
  • CCL4 (MIP-1B)-Biotin
  • CCL5 (Rantes)-Biotin
  • CCL7 (MCP-3)-Biotin
  • CCL14 (HCC-1)-Biotin
  • CCL19 (MIP-3B)-Biotin
  • CCL28 (MEC)-Biotin
  • CXCL8 (IL-8)-Biotin
  • CXCL12 (SDF-1a)-Biotin​
​Support: [email protected]
For Research use only, Not for use in Humans.
Picture
​ChemoTactics, Inc
6076 Corte del Cedro Carlsbad,
​CA 92011 USA
Phone: +1-(858)-412-0485
​Email: [email protected]
Chemokine Products
Biotinylated Chemokines
Unconjugated Chemokines
Streptavidin Conjugates
Research Services
Resources​
Published Applications
Protocols
​Blog
About
​
Contact
Made in the USA
  • Chemokine Products
    • Biotinylated Chemokines >
      • Biotinylated CCL2 (MCP-1)
      • Biotinylated CCL3 (MIP-1α)
      • Biotinylated CCL4 (MIP-1β)
      • Biotinylated CCL5 (Rantes)
      • Biotinylated CCL7 (MCP-3)
      • Biotinylated CCL14 (HCC-1)
      • Biotinylated CCL19 (MIP-3β)
      • Biotinylated CCL28 (MEC)
      • Biotinylated CXCL8 (IL-8)
      • Biotinylated CXCL12 (SDF-1α)
    • Unmodified Chemokines >
      • CCL2 (MCP-1)
      • CCL3 (MIP-1α)
      • CCL4 (MIP-1β)
      • CCL5 (RANTES)
      • CCL7 (MCP-3)
      • CCL14 (HCC-1)
      • CCL19 (MIP-3β)
      • CCL27 (CTACK)
      • CCL28 (MEC)
      • CXCL8 (IL-8)
      • CXCL10 (IP-10)
      • CXCL12 (SDF-1α)
  • Streptavidin Conjugates
  • Services
  • Resources
    • Published Applications Biotinylated CXCL12 and CCL5
    • What are Chemokines?
    • Chemokine Protocols >
      • Flow Cytometry with Biotinylated Chemokines
      • ELISA with Biotinylated Chemokines
      • Transwell Migration Assay
    • Blog
  • About
    • Contact