Biotinylated Human CCL5 (Rantes)
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SPECIFICATIONS
Source: E. coli derived Accession # P13501 (24-91) Modification: Biotin at C-Terminal Formulation: Lyophilized Carrier Protein: None Predicted Molecular Mass: 10,269.7258 Da Extinction Coefficient: 18,020 M-1 cm-1 Actual Molecular Mass: 10,269.7258 Da by ESI Mass Spec Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method Purity: > 97% by SDS PAGE PREPARATION AND STORAGE Reconstitution: Spin sample prior to reconstitution. Recommended concentration of 100μg/mL in sterile water. Shipping: Room Temp Stability and Storage: Avoid repeated freeze-thaw cycles. • 12 months from date of receipt, -20 to -70 °C as supplied. • ChemoTactics suggests using immediately after reconstitution. • 1 month, -20 to -70 °C under sterile conditions after reconstitution. |
Migration Assay: Cells expressing recombinant CCR5 were assayed for migration through a transwell filter at various concentrations of WT Rantes or Biotinylated Rantes. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).
Migration Assay Protocol 
Activity: EC50 = 0.25-0.50nM determined by Migration Assay of recombinant CCR5 containing cells
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For bulk orders or custom sizes, please contact us and we can provide this for you.
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REFERENCES
1. "The Cytokine Nicotinamide Phosphoribosyltransferase (eNAMPT; PBEF; Visfatin) Acts as a Natural Antagonist of C-C Chemokine Receptor Type 5 (CCR5)"
Torretta S., Colombo G., Travelli C, Boumya S., Lim D., Genazzani A., Brolla A.
Cells 2020 Feb; 9(2):496. doi 10.3390/cells9020496 PMID: 32098202
2. “RANTES: a versatile and controversial chemokine”
Appay V., Rowland-Jones S.L.
Trends Immunol 22:83-87 (2001)
3. “Identification of RANTES, MIP-1, and MIP-1[1] as the major HIV-suppressive factors produced by CD8+T cells”
Cocchi F., De Vico A.L., Garzino-Demo A., Arya S.K., Gallo R.C., Lusso P.
Science 270:1811-1815 (1995)
4. A human T cell-specific molecule is a member of a new gene family
Schall T.J., Jongstra J., Dyer B.J., Jorgensen J., Clayberger C., Davis M.M., Krensky A.M.
J Immunol 141:1018-1025 (1988)
5. “Engineering the glycosaminoglycan-binding affinity, kinetics and oligomerization behavior of RANTES: a tool for generating chemokine-based glycosaminoglycan antagonists”
Brandner B., Rek A., Diedrichs-Möhring M., Wildner G., Kungl A.J.
Protein Eng Des Sel. 22:367-373 (2009)
1. "The Cytokine Nicotinamide Phosphoribosyltransferase (eNAMPT; PBEF; Visfatin) Acts as a Natural Antagonist of C-C Chemokine Receptor Type 5 (CCR5)"
Torretta S., Colombo G., Travelli C, Boumya S., Lim D., Genazzani A., Brolla A.
Cells 2020 Feb; 9(2):496. doi 10.3390/cells9020496 PMID: 32098202
2. “RANTES: a versatile and controversial chemokine”
Appay V., Rowland-Jones S.L.
Trends Immunol 22:83-87 (2001)
3. “Identification of RANTES, MIP-1, and MIP-1[1] as the major HIV-suppressive factors produced by CD8+T cells”
Cocchi F., De Vico A.L., Garzino-Demo A., Arya S.K., Gallo R.C., Lusso P.
Science 270:1811-1815 (1995)
4. A human T cell-specific molecule is a member of a new gene family
Schall T.J., Jongstra J., Dyer B.J., Jorgensen J., Clayberger C., Davis M.M., Krensky A.M.
J Immunol 141:1018-1025 (1988)
5. “Engineering the glycosaminoglycan-binding affinity, kinetics and oligomerization behavior of RANTES: a tool for generating chemokine-based glycosaminoglycan antagonists”
Brandner B., Rek A., Diedrichs-Möhring M., Wildner G., Kungl A.J.
Protein Eng Des Sel. 22:367-373 (2009)
