Biotinylated Human CCL28 (MEC)
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SPECIFICATIONS
Source: E. coli derived Accession # Q9NRJ3 (23-127) Modification: Biotin at C-Terminal Formulation: Lyophilized Carrier Protein: None Predicted Molecular Mass: 14,490.7111 Da Extinction Coefficient: 14,300 M-1 cm-1 Actual Molecular Mass: 14,490.7111 Da by ESI Mass Spec Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method Purity: > 97% by SDS PAGE PREPARATION AND STORAGE Reconstitution: Spin sample prior to reconstitution. Recommended concentration of 100μg/mL in sterile water. Shipping: Room Temp Stability and Storage: Avoid repeated freeze-thaw cycles. • 12 months from date of receipt, -20 to -70 °C as supplied. • ChemoTactics suggests using immediately after reconstitution. • 1 month, -20 to -70 °C under sterile conditions after reconstitution. |
Migration Assay: Cells expressing recombinant CCR10 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated MEC. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).
Migration Assay Protocol 
Activity: EC50 = 40-60 nM determined by migration assay with cells expressing recombinant CCR10
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For bulk orders or custom sizes, please contact us and we can provide this for you.
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REFERENCES
1. “A novel chemokine ligand for CCR10 and CCR3 expressed by epithelial cells in mucosal tissues”
Pan et al.
J. Immunol. 165: 2943–2949 (2000)
2. Identification of a novel chemokine (CCL28), which binds CCR10 (GPR2).
Wang et al.
J. Biol. Chem. 275: 22313–22323 (2000)
3. “The Mucosae-Associated Epithelial Chemokine (MEC/CCL28) Modulates Immunity in HIV Infection”
Castelletti E. at al.
PLoS ONE 2:e969 (2007)
1. “A novel chemokine ligand for CCR10 and CCR3 expressed by epithelial cells in mucosal tissues”
Pan et al.
J. Immunol. 165: 2943–2949 (2000)
2. Identification of a novel chemokine (CCL28), which binds CCR10 (GPR2).
Wang et al.
J. Biol. Chem. 275: 22313–22323 (2000)
3. “The Mucosae-Associated Epithelial Chemokine (MEC/CCL28) Modulates Immunity in HIV Infection”
Castelletti E. at al.
PLoS ONE 2:e969 (2007)
Chemokine Products
Support: [email protected]
For Research use only, Not for use in Humans.
For Research use only, Not for use in Humans.
