Human Biotin-CCL3 (MIP-1α)
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Stock Sizes: 2ug, 10ug, 50ug, 100ug, and 1mg
Also available in custom sizes, email for a custom quote. |
BACKGROUND
Macrophage inflammatory proteins 1-α (MIP1α)(CCL3) binds to cell surface receptors CCR1 and CCR5. It is proinflammatory, leading to chemotaxis and activation of immune cells. It also inhibits proliferation of hematopoietic stem cells. Moreover, by binding to one of the HIV coreceptors, CCR5, it suppresses HIV infection.
Macrophage inflammatory proteins 1-α (MIP1α)(CCL3) binds to cell surface receptors CCR1 and CCR5. It is proinflammatory, leading to chemotaxis and activation of immune cells. It also inhibits proliferation of hematopoietic stem cells. Moreover, by binding to one of the HIV coreceptors, CCR5, it suppresses HIV infection.
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SPECIFICATIONS
Source: E. coli derived Accession # P10147 (24-92) Modification: Biotin at C-Terminal Formulation: Lyophilized Carrier Protein: None Predicted Molecular Mass: 10,135.342 Da Extinction Coefficient: 15,460 M-1 cm-1 Actual Molecular Mass: 10,135.342 Da by ESI Mass Spec Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method Purity: > 97% by SDS PAGE |
PREPARATION AND STORAGE
Reconstitution: Spin sample prior to reconstitution. Recommended concentration of 100μg/mL in sterile water. Shipping: Room Temp Stability and Storage: Avoid repeated freeze-thaw cycles. • 12 months from date of receipt, -20 to -70 °C as supplied. • ChemoTactics suggests using immediately after reconstitution. • 1 month, -20 to -70 °C under sterile conditions after reconstitution. |
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Migration Assay: Cells expressing recombinant CCR5 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated MIP-1α. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).
Migration Assay Protocol 
Activity: EC50 = 1.1-2.6nM determined by Migration Assay of recombinant CCR5 containing cells
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Biotin-CCL3 (MIP-1α) |
REFERENCES
1. “Macrophage inflammatory protein-1” Menten P., Wuyts A., Van Damme J. Cytokine Growth Factor Rev. 13:455-481(2002)
2. “Identification of RANTES, MIP-1, and MIP-1[1] as the major HIV-suppressive factors produced by CD8+T cells”
Cocci F., De Vico A.L., Garzino-Demo A., Arya S.K., Gallo R.C., Lusso P. Science 270:1811-1815 (1995)
3. “Identification and characterization of an inhibitor of haemopoietic stem cell proliferation”
Graham G.J., Wright E.G., Hewick R., Wolpe S.D., Wilkie N.M., Donaldson D., Lorimore S., Pragnell I.B. Nature 344:442-444 (1990)
4. “Mitogenic activation of human T cells induces two closely related genes which share structural similarities with a new family of secreted factors”
Zipfel P.F., Balke J., Irving S.G., Kelly K., Siebenlist U. J Immunol 142:1582-1590 (1989)
1. “Macrophage inflammatory protein-1” Menten P., Wuyts A., Van Damme J. Cytokine Growth Factor Rev. 13:455-481(2002)
2. “Identification of RANTES, MIP-1, and MIP-1[1] as the major HIV-suppressive factors produced by CD8+T cells”
Cocci F., De Vico A.L., Garzino-Demo A., Arya S.K., Gallo R.C., Lusso P. Science 270:1811-1815 (1995)
3. “Identification and characterization of an inhibitor of haemopoietic stem cell proliferation”
Graham G.J., Wright E.G., Hewick R., Wolpe S.D., Wilkie N.M., Donaldson D., Lorimore S., Pragnell I.B. Nature 344:442-444 (1990)
4. “Mitogenic activation of human T cells induces two closely related genes which share structural similarities with a new family of secreted factors”
Zipfel P.F., Balke J., Irving S.G., Kelly K., Siebenlist U. J Immunol 142:1582-1590 (1989)
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For Research use only, Not for use in Humans.
For Research use only, Not for use in Humans.
