Biotin-CCL2 (MCP-1)
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$130.00
$104.00 - $11,877.00
$104.00
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Monocyte chemoattractant protein-1 (MCP-1/CCL2) is produced by many cell types at sites of inflammation. It regulates chemotaxis and transendothielial migration of monocytes, as well as memory T cells and natural killer cells by interacting with their membrane surface receptor CCR2. MCP-1 has also been implicated in a number of disease states, such as rheumatoid arthritis, atherosclerosis, autoimmune diseases, tumor progression, and HIV infection.
Biotinylated CCL2 is made using the enzymatic method, which has several advantages over chemical biotinylation methods. The attachment of biotin at a specific lysine residue within the C-terminal avi tag sequence is nearly 100% complete, and leads to a modified chemokine with functionalities comparable to those of the unmodified CCL2 in migration assay. Combining with avidin analogues conjugated to various fluorescent labels, biotinylated CCL2 is useful in studies on receptor identification, distribution, chemokine binding, and other cellular assays. They serve as great tools in visualization and quantification, and replace the needs for radioactively labeled chemokines.
Biotinylated CCL2 is made using the enzymatic method, which has several advantages over chemical biotinylation methods. The attachment of biotin at a specific lysine residue within the C-terminal avi tag sequence is nearly 100% complete, and leads to a modified chemokine with functionalities comparable to those of the unmodified CCL2 in migration assay. Combining with avidin analogues conjugated to various fluorescent labels, biotinylated CCL2 is useful in studies on receptor identification, distribution, chemokine binding, and other cellular assays. They serve as great tools in visualization and quantification, and replace the needs for radioactively labeled chemokines.
