CCL2 (MCP-1)
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Monocyte chemoattractant protein-1 (MCP-1) (CCL2) is produced by many cell types at sites of inflammation. It regulates chemotaxis and transendothielial migration of monocytes, as well as memory T cells and natural killer cells by interacting with their membrane surface receptor CCR2. MCP-1 has also been implicated in a number of disease states, such as rheumatoid arthritis, atherosclerosis, autoimmune diseases, tumor progression, and HIV infection.
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SPECIFICATIONS
Source: E. coli derived Accession # P13500 (24-99) Modification: None Actual Molecular Mass (Mass Spec): Mass spec data confirmed the expected mass of 8.666 kDa Predicted Molecular Mass: 8.666 kDa Extinction Coefficient: 8,730 M-1 cm-1 Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method Protein Sequence:QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQK WVQDSIDHLDKQTQTPKT Activity: EC50 = 0.066nM determined by Migration Assay with cells expressing recombinant CCR2 Formulation: Lyophilized Carrier Protein: None Purity: > 97% by SDS PAGE |
Migration Assay: Cells expressing recombinant CCR2 were assayed for migration through a transwell filter at various concentrations of MCP-1. Responses are expressed as the % of total input cells.
Migration Assay Protocol 
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